PEF Production Pipeline
The PEF production pipeline provides a quick snapshot of the services we offer to help fast-track your research. From the Gene-to-Protein complete package to any stage in-between, we can customise to suit your project.Contact us to discuss how we can help support your research. Download the complete list as a pdf here.
2. Molecular Cloning
Molecular Cloning | |
Gene synthesis |
Generate the DNA sequence of a gene of interest. Codon optimisation (optional) for the host expression system. |
Expression construct |
Clone the gene of interest into chosen expression vector. DNA sequence verification of cloned construct. |
Mutagenesis |
Single or multiple nucleotide base change e.g. amino acid substitution, insert/delete restriction enzyme site, stop codon and small tag. DNA sequence verification of cloned construct. |
Plasmid production |
Production and purification of plasmid DNA from mini- to mega-prep scale. |