The PEF production pipeline provides a quick snapshot of the services we offer to help fast-track your research.  From the Gene-to-Protein complete package to any stage in-between, we can customise to suit your project.Contact us to discuss how we can help support your research. Download the complete list as a pdf here.

3. Small-scale Expression Screening

 Small-scale Expression Screening

 E. coli

Expression screen in multiple conditions.  Each construct tested in two different expression strains, two temperatures and two media. Analyse expression and Ni-NTA binding by SDS-PAGE. Resin-binding assay for polyhistidine-tagged constructs only. Determine optimal expression condition.

 Pichia pastoris

Expression screen of 10 yeast clones per construct. Analyse expression and Ni-NTA binding by SDS-PAGE. Resin-binding assay for polyhistidine-tagged constructs only. Determine optimal expression condition.

 Insect cell/baculovirus

Expression screen in multiple conditions. Each construct tested in two cell lines and at two temperatures. Analyse expression and Ni-NTA binding by SDS-PAGE and/or Western blot. Resin-binding assay for polyhistidine-tagged constructs only. Determine optimal expression condition.

 Mammalian cell

Transient expression screen in two cell lines. Analyse expression and Ni-NTA binding by SDS-PAGE and/or Western blot. Resin-binding assay for polyhistidine-tagged constructs only. Determine optimal expression condition

 In vitro cell-free

Expression screen using Leishmania tarentolae lysate. Analyse expression by SDS-PAGE.