The PEF production pipeline provides a quick snapshot of the services we offer to help fast-track your research.  From the Gene-to-Protein complete package to any stage in-between, we can customise to suit your project.Contact us to discuss how we can help support your research. Download the complete list as a pdf here.

4. Large-scale Protein Expression

Large-scale Protein Expression

E. coli or Pichia pastorisexpression in shake flask

Expression culture at ≥ 1 L in shake flasks. Cells and/or supernatant collected at optimal time of harvest. Analysis by SDS-PAGE.

Insect cell/baculovirus expression in shake flask

Scale-up of virus stock. Expression culture at ≥ 1 L in shake flasks.  Cells and/or supernatant collected at optimal time of harvest. Analysis by SDS-PAGE and/or Western blot.

Mammalian cell transient expression in shake flask

Maxi-prep plasmid production. Expression culture at ≥ 1 L in shake flasks. Cells and/or supernatant collected at optimal time of harvest. Analysis by SDS-PAGE and/or Western blot.

E. coli or Pichia pastorisfermentation in bioreactor

Fermentation in a stirred tank bioreactor, from 2 L to 20 L. Analysis by SDS-PAGE.

Insect or mammalian cell expression in bioreactor

Expression in a WAVE bioreactor, from 1 L to 25 L. Analysis by SDS-PAGE and/or Western blot.