PEF Production Pipeline
The PEF production pipeline provides a quick snapshot of the services we offer to help fast-track your research. From the Gene-to-Protein complete package to any stage in-between, we can customise to suit your project.Contact us to discuss how we can help support your research. Download the complete list as a pdf here.
4. Large-scale Protein Expression
Large-scale Protein Expression | |
E. coli or Pichia pastorisexpression in shake flask |
Expression culture at ≥ 1 L in shake flasks. Cells and/or supernatant collected at optimal time of harvest. Analysis by SDS-PAGE. |
Insect cell/baculovirus expression in shake flask |
Scale-up of virus stock. Expression culture at ≥ 1 L in shake flasks. Cells and/or supernatant collected at optimal time of harvest. Analysis by SDS-PAGE and/or Western blot. |
Mammalian cell transient expression in shake flask |
Maxi-prep plasmid production. Expression culture at ≥ 1 L in shake flasks. Cells and/or supernatant collected at optimal time of harvest. Analysis by SDS-PAGE and/or Western blot. |
E. coli or Pichia pastorisfermentation in bioreactor |
Fermentation in a stirred tank bioreactor, from 2 L to 20 L. Analysis by SDS-PAGE. |
Insect or mammalian cell expression in bioreactor |
Expression in a WAVE bioreactor, from 1 L to 25 L. Analysis by SDS-PAGE and/or Western blot. |