The PEF production pipeline provides a quick snapshot of the services we offer to help fast-track your research.  From the Gene-to-Protein complete package to any stage in-between, we can customise to suit your project.Contact us to discuss how we can help support your research. Download the complete list as a pdf here.

5. Protein Purification

Protein Purification

Primary purification from cell pellet

Sample extraction followed by affinity chromatography (e.g. IMAC, GST, MBP, Strep(ll), heparin). Analysis by SDS-PAGE.

Primary purification from supernatant

Tangential flow ultrafiltration for sample concentration and buffer exchange.  Primary purification by affinity chromatography (e.g. IMAC, GST, MBP, Strep(ll), heparin, Protein A). Analysis by SDS-PAGE.

Secondary or polishing purification

Secondary or polishing purification to improve product purity by chromatography techniques such as affinity, ion exchange, hydrophobic interaction or gel filtration.  Analysis by SDS PAGE.

Fusion tag removal

Scout optimal protease cleavage condition to remove fusion tag from protein. Proteases used are Enterokinase, Factor Xa, HRV-3C, SUMO, TEV or Thrombin. Chromatography to recover untagged protein. Analysis by SDS PAGE.

Endotoxin removal

Removal of endotoxin from purified protein. Analysis by SDS-PAGE and endotoxin determination by FDA-licensed LAL cartridges.