PEF Production Pipeline
The PEF production pipeline provides a quick snapshot of the services we offer to help fast-track your research. From the Gene-to-Protein complete package to any stage in-between, we can customise to suit your project.Contact us to discuss how we can help support your research. Download the complete list as a pdf here.
5. Protein Purification
Protein Purification | |
Primary purification from cell pellet |
Sample extraction followed by affinity chromatography (e.g. IMAC, GST, MBP, Strep(ll), heparin). Analysis by SDS-PAGE. |
Primary purification from supernatant |
Tangential flow ultrafiltration for sample concentration and buffer exchange. Primary purification by affinity chromatography (e.g. IMAC, GST, MBP, Strep(ll), heparin, Protein A). Analysis by SDS-PAGE. |
Secondary or polishing purification |
Secondary or polishing purification to improve product purity by chromatography techniques such as affinity, ion exchange, hydrophobic interaction or gel filtration. Analysis by SDS PAGE. |
Fusion tag removal |
Scout optimal protease cleavage condition to remove fusion tag from protein. Proteases used are Enterokinase, Factor Xa, HRV-3C, SUMO, TEV or Thrombin. Chromatography to recover untagged protein. Analysis by SDS PAGE. |
Endotoxin removal |
Removal of endotoxin from purified protein. Analysis by SDS-PAGE and endotoxin determination by FDA-licensed LAL cartridges. |