Mesenchymal Stem Cell Differentiation, Microfluidics and Proteomics

Harish Padmanabhan's research involves trying to differentiate human mesenchymal stem cells into osteoblasts by targetting pathways relevant to osteogenesis. Next, using the microbioreactor array platform developed in the lab, he'll be looking at the individual and the interaction effects of different small molecules/growth factors (that activate/inhibit signalling pathways) on osteogenic differentiation and the impact of autocrine/paracrine signalling during osteogenesis. In the next part of his project, he'll be designing a new microfluidic device that has the capability to isolate the cell secretome in real time and using a proteomics approach identify the factors secreted by the cell in response to the different factors. Finally, he'll be looking at the complex interplay between cell - ECM and cell - soluble factors (growth factors/small molecules) interactions during osteogenesis

Harish is currently doing his PhD (Final year) at the Australian Institute of Bioengineering and Nanotechnology under the guidance of Prof. Justin Cooper-White and Prof. Ernst Wolvetang. ​He has completed his Masters in Biotechnology from The University of Queensland and Bachelors in Engineering (Biotechnology) from Visvesvaraya Technological University (India). Achievements: Deans Commendation for 2 Semesters during Masters

Key Publications

  1. ​Frith JE, Titmarsh DM, Padmanabhan H, Cooper-White JJ (2013) Microbioreactor Array Screening of Wnt Modulators and Microenvironmental Factors in Osteogenic Differentiation of Mesenchymal Progenitor Cells. PLoS ONE 8(12): e82931. doi:10.1371/journal.pone.0082931
  2. Wang, Hui, Rauf, Sakandar, Padmanabhan, Harish and Dimitrov, Krassen (2013) Nanodumbbells as multi-functional diagnosis probes. Journal of Nanoparticle Research, 15 5: 1633.1-1633.9. doi:10.1007/s11051-013-1633-2