α-chymotrypsin modifications

​Tommaso's project is about determining the effect of different modifications on the original α-chymotrypsin artificial catalytic triad on the free energy plot of the catalytic cycle, specifically the change in energy for the two rate-determining transition states. (They are very close in energy difference from the reactants, so if one is lowered significantly by a modification, the other will be rate determining). This is determined through DFT transition state studies.