Dr Eugene Wee
Postdoctoral Research Fellow
Australian Institute for Bioengineering and Nanotechnology
+61 7 334 64176
Book Chapter
Wee, Eugene J. H., Shiddiky, Muhammad J. A. and Trau, Matt (2013). Detecting DNA methylation for cancer diagnostics and prognostics. In Muhammad J. A. Shiddiky, Eugene J. H. Wee, Sakandar Rauf and Matt Trau (Ed.), Microfluidics, nanotechnology and disease biomarkers for personalized medicine applications (pp. 117-132) New York, NY, United States: Nova Publishers.
Journal Articles
Zou, Yiping, Mason, Michael Glenn, Wang, Yuling, Wee, Eugene, Turni, Conny, Blackall, Patrick J., Trau, Matt and Botella, Jose Ramon (2017) Nucleic acid purification from plants, animals and microbes in under 30 seconds. PLoS Biology, 15 11: e2003916. doi:10.1371/journal.pbio.2003916
Ng, Benjamin Y. C., Wee, Eugene J. H., Woods, Kyra, Anderson, Will, Antaw, Fiach, Tsang, Hennes Z. H., West, Nicholas P. and Trau, Matt (2017) Isothermal point mutation detection: toward a first-pass screening strategy for multidrug-resistant tuberculosis. Analytical Chemistry, 89 17: 9017-9022. doi:10.1021/acs.analchem.7b01685
Koo, Kevin M., Wee, Eugene J. H., Wang, Yuling and Trau, Matt (2017) EEnabling miniaturised personalised diagnostics: from lab-on-a-chip to lab-in-a-drop. Lab On a Chip, 17 19: 3200-3220. doi:10.1039/c7lc00587c
Koo, Kevin M., Wee, Eugene J. H. and Trau, Matt (2017) High-speed biosensing strategy for non-invasive profiling of multiple cancer fusion genes in urine. Biosensors and Bioelectronics, 89 2: 715-720. doi:10.1016/j.bios.2016.11.024
Wang, Jing, Koo, Kevin M., Wee, Eugene J. H., Wang, Yuling and Trau, Matt (2017) A nanoplasmonic label-free surface-enhanced Raman scattering strategy for non-invasive cancer genetic subtyping in patient samples. Nanoscale, 9 10: 3496-3503. doi:10.1039/c6nr09928a
Lau, Han Yih, Wu, Haoqi, Wee, Eugene J. H., Trau, Matt, Wang, Yuling and Botella, Jose R. (2017) Specific and sensitive isothermal electrochemical biosensor for plant pathogen DNA detection with colloidal gold nanoparticles as probes. Scientific Reports, 7 1: 38896. doi:10.1038/srep38896
Mok, Ellie, Wee, Eugene, Wang, Yuling and Trau, Matt (2016) Comprehensive evaluation of molecular enhancers of the isothermal exponential amplification reaction. Scientific Reports, 6 37837. doi:10.1038/srep37837
Koo, Kevin M., McNamara, Benjamin, Wee, Eugene J. H., Wang, Yuling and Trau, Matt (2016) Rapid and sensitive fusion gene detection in prostate cancer urinary specimens by label-free surface-enhanced raman scattering. Journal of Biomedical Nanotechnology, 12 9: 1798-1805. doi:10.1166/jbn.2016.2294
Lau, Han Yih, Wang, Yuling, Wee, Eugene J. H., Botella, Jose R. and Trau, Matt (2016) Field demonstration of a multiplexed point-of-care diagnostic platform for plant pathogens. Analytical Chemistry, 88 16: 8074-8081. doi:10.1021/acs.analchem.6b01551
Koo, Kevin M., Wee, Eugene J. H., Mainwaring, Paul N. and Trau, Matt (2016) A simple, rapid, low-cost technique for naked-eye detection of urine-isolated TMPRSS2:ERG gene fusion RNA. Scientific reports, 6 . doi:10.1038/srep30722
Wee, Eugene J. H., Wang, Yuling, Simon, Chang-Hao Tsao and Trau, Matt (2016) Simple, sensitive and accurate multiplex detection of clinically important melanoma DNA mutations in circulating tumour DNA With SERS nanotags. Theranostics, 6 10: 1506-1513. doi:10.7150/thno.15871
Koo, Kevin, Wee, Eugene and Trau, Matt (2016) Colorimetric TMPRSS2-ERG gene fusion detection in prostate cancer urinary samples via recombinase polymerase amplification. Theranostics, 6 9: 1415-1424. doi:10.7150/thno.15250
Wee, Eugene J. H. and Trau, Matt (2016) Simple isothermal strategy for multiplexed, rapid, sensitive, and accurate miRNA detection. ACS Sensors, 1 6: 670-675. doi:10.1021/acssensors.6b00105
Wang, Yuling, Wee, Eugene J. H. and Trau, Matt (2016) Accurate and sensitive total genomic DNA methylation analysis from sub-nanogram input with embedded SERS nanotags. Chemical Communications, 52 17: 3560-3563. doi:10.1039/C6CC00547K
Koo, Kevin M., Wee, Eugene J. H., Mainwaring, Paul N., Wang, Yuling and Trau, Matt (2016) Toward precision medicine: a cancer molecular subtyping nano-trategy for RNA biomarkers in tumor and urine. Small, 12 45: 6233-6242. doi:10.1002/smll.201602161
Ng, Benjamin Y. C., Wee, Eugene J. H., West, Nicholas P. and Trau, Matt (2015) Naked-eye colorimetric and electrochemical detection of Mycobacterium tuberculosis—toward rapid screening for active case finding. ACS Sensors, 1 2: 173-178. doi:10.1021/acssensors.5b00171
Ng, Benjamin Y. C., Xiao, Wei, West, Nicholas P., Wee, Eugene J. H., Wang, Yuling and Trau, Matt (2015) Rapid, Single-Cell Electrochemical Detection of Mycobacterium tuberculosis Using Colloidal Gold Nanoparticles. Analytical Chemistry, 87 20: 10613-10618. doi:10.1021/acs.analchem.5b03121
Ng, Benjamin Y.C., Wee, Eugene J. H., West, Nicholas P. and Trau, Matt (2015) Rapid DNA detection of Mycobacterium tuberculosis-towards single cell sensitivity in point-of-care diagnosis. Scientific Reports, 5 15027.1-15027.7. doi:10.1038/srep15027
Wee, Eugene J. H., Ngo, Thu Ha and Trau, Matt (2015) A simple bridging flocculation assay for rapid, sensitive and stringent detection of gene specific DNA methylation. Scientific Reports, 5 Art No.: 15028: . doi:10.1038/srep15028
Wee, Eugene J. H., Ngo, Thu Ha and Trau, Matt (2015) Colorimetric detection of both total genomic and loci-specific DNA methylation from limited DNA inputs. Clinical Epigenetics, 7 1: 65. doi:10.1186/s13148-015-0100-6
Wee, E. J. H., Lau, H. Y., Botella, J. and Trau, M. (2015) Re-purposing bridging flocculation for on-site, rapid, qualitative DNA detection in resource-poor settings. Chemical Communications, 51 27: 5828-5831. doi:10.1039/C4CC10068A
Wang, Yuling, Wee, Eugene J. H. and Trau, Matt (2015) Highly sensitive DNA methylation analysis at CpG resolution by surface-enhanced Raman scattering via ligase chain reaction. Chemical Communications, 51 54: 10953-10956. doi:10.1039/c5cc03921e
Wee, Eugene J. H., Rauf, Sakandar, Shiddiky, Muhammad J. A., Dobrovic, Alexander and Trau, Matt (2014) DNA ligase-based strategy for quantifying heterogeneous DNA methylation without sequencing. Clinical Chemistry, 61 1: 163-171. doi:10.1373/clinchem.2014.227546
Wee, Eugene J. H. and Trau, Matt (2014) Enzyme catalysis: evolution made easy. Nature Chemistry, 6 9: 756-757. doi:10.1038/nchem.2046
Wee, Eugene J. H. and Trau, Matt (2014) Measuring whole genome methylation via oxygen channelling chemistry. Chemical Communications, 50 74: 10894-10896. doi:10.1039/C4CC04623D
Koo, Kevin M., Wee, Eugene, Rauf, Sakandar, Shiddiky, Muhammad J. A. and Trau, Matt (2014) Microdevices for detecting locus-specific DNA methylation at CpG resolution. Biosensors and Bioelectronics, 56 278-285. doi:10.1016/j.bios.2014.01.029
Shiddiky, Muhammad J.A., Wee, Eugene J.H., Rauf, Sakandar and Trau, Matt (2013) Preface. Microfluidics, Nanotechnology and Disease Biomarkers for Personalized Medicine Applications, .
Wee, Eugene J. H., Rauf, Sakandar, Koo, Kevin, Shiddiky, Muhammad J. A. and Trau, Matt (2013) mu-eLCR: a microfabricated device for electrochemical detection of DNA base changes in breast cancer cell lines. Lab on a Chip, 13 22: 4385-4391-4385-4391. doi:10.1039/C3LC50528F
Wee, Eugene J. H., Shiddiky, Muhammad J. A., Brown, Melissa A. and Trau, Matt (2012) eLCR: electrochemical detection of single DNA base changes via ligase chain reaction. Chemical Communications, 48 98: 1214-1216. doi:10.1039/C2CC35841G
Wee, E.J.H., Peters, K., Nair, S.S., Hulf, T., Stein, S., Wagner, S., Bailey, P., Lee, S.Y., Qu, W.J., Brewster, B., French, J.D., Dobrovic, A., Francis, G.D., Clark, S.J. and Brown, M.A. (2012) Mapping the regulatory sequences controlling 93 breast cancer-associated miRNA genes leads to the identification of two functional promoters of the Hsa-mir-200b cluster, methylation of which is associated with metastasis or hormone receptor status in adva. Oncogene, 31 38: 4182-4195. doi:10.1038/onc.2011.584
Yeo, Dawn Su-Yin, Ng, Sock-Hoon, Liaw, Chin-Wen, Ng, Ley-Moy, Wee, Eugene Jing-Hui, Lim, Elizabeth Ai-Sim, Seah, Shirely Lay-Kheng, Wong, Wai-Kwan, Lim, Chee-Wee, Sugrue, Richard J. and Tan, Boon-Huan (2009) Molecular characterization of low pathogenic avian influenza viruses, isolated from food products imported into Singapore. Veterinary Microbiology, 138 3-4: 304-317. doi:10.1016/j.vetmic.2009.04.025
Conference Papers
Koo, K., Wee, E., Mainwaring, P., Wang, Y. and Trau, M. (2016). Towards precision medicine: A cancer molecular subtyping nano-strategy for RNA biomarkers in tumor and urine. In: 28th EORTC-NCI-AACR Symposium on Molecular Targets and Cancer Therapeutics, Munich Germany, (S33-S33). November 29 - December 2 2016.
Wee, Eugene J. H., Koo, Kevin M., Mainwaring, Paul N. and Trau, Mattias (2015). Towards rapid and cost-effective point-of-care detection of TMPRSS2:ERG fusion transcripts in urine via a novel methodology. In: 2015 ASCO Annual Meeting Abstracts. Annual Meeting of the American Society of Clinical Oncology (ASCO), Chicago, IL United States, (). 29 May-02 June 2015.
Thesis
Wee, Jing Hui (Eugene) (2012). Detection and quantification of miRNA promoter DNA methylation as prognostic biomarkers for breast cancer PhD Thesis, School of Chemistry and Molecular Biosciences, The University of Queensland.