Study

Lighting up snake venom proteins: fluorescently tag procoagulant  to monitor movement at wound sites during blood clotting.

  • Design and construct eGFP tagged procoagulant 
  • Express and purify recombinant eGFP tagged procoagulant 
  • Use in vitro biochemical assays to assess the activity of recombinant eGFP-procoagulant 
  • Use blood coagulation assays to assess the activity of recombinant eGFP-procoagulant .
  • Investigate efficacy of eGFP-procoagulant  hydrogel based wound dressings in models of hemostasis
  • Monitor the movement of eGFP-procoagulant  in hydrogel based wound dressing in models of hemostasis

 

 

Tackling Coagulopathy (uncontrolled bleeding), a major cause of trauma-related deaths using hydrogel based wound dressings

  • Investigate bioactive molecules for effective control of coagulopathy in addition to procoagulant 
  • Investigate efficacy of candidate bioactive molecules in models of hemostasis
  • Incorporation of bioactive molecules into polyisocyanopeptide based hydrogel bio-functionalised with procoagulant 
  • Investigate efficacy of blood coagulation of hydrogel wound dressings containing candidate bioactive molecules.

 

Really get to know your snake venom proteins: investigation of procoagulant ’s blood clotting activity and the effect of the disintegrin and ADAM domains, as well as the effect of metal ions on this pro-coagulant metalloprotease.

  • Investigate effects if various metal ions on recombinant procoagulant ’s catalytic activity
  • Design and construct truncated procoagulant  recombinant proteins lacking disintegrin and ADAM C-terminal domains
  • Express and purify recombinant truncated procoagulant 
  • in vitro assay for effective therapeutic control of bleeding using truncated procoagulant 
  • compare kinetic activity of full-length and truncated procoagulant