Project Summary

Visualization of focal adhesions and cellular activity as a function of applied force by extracellular matrix (ECM) can provide crucial information about the cells response to mechanical manipulation and ultimately allows us to obtain more detailed understanding of mechanotransduction. The aim of this project is to develop a time resolved, 3D imaging and ultimately super resolution capable combined confocal microscope-rheometer setup, that can obtain simultaneous readout of response from single cells (fluorescence microscopy) and the visco-elastic properties (rheology) of ECM that surrounds the cells.

Immune Synapse Formation
Schematic representation of the setup, where behaviour of individual cells inside biomimetic polyisocyanide hydrogel is monitored using combined confocal fluorescence microscope-rheometer.

 

Research Group

Rowan Group

Keywords

Fluorescence microscopy, Confocal microscopy, Rheology, 3D live cell imaging

 

 

Project members

Lead Investigator


Professor Alan Rowan

AIBN Institute Director
Senior Group Leader
Rowan Group

Researchers Involved


Dr Petri Turunen

Postdoctoral Research Fellow
Rowan Group

Dr Jan Lauko

Postdoctoral researcher - Group operations manager
Rowan group