Project Summary

Clostridial toxoid production can be enhanced through the use of systems biology.

Toxoid vaccines are used routinely in the livestock industry to prevent animal-disease caused by pathogenic clostridia. Vaccines are produced using batch fermentation processes, which have undergone limited optimization over the past five decades. Low titres and frequent batch failures greatly affect capital utilization and represent a significant cost factor. This project uses high-throughput chemistry (omics) to produce molecular maps of fermentation, which can be used to design a new generation of superior fermentation processes.

Achieving the aims is of direct relevance to diagnose causes of batch failure (e.g., raw material failures) and engineer productive, high density fermentation which are used to produce toxins to prevent multiple animal diseases.


Research Group

Marcellin Group


Clostridium; Zoetis; pathogenic clostridia; tetanus

Project members

Lead Investigator

Professor Esteban Marcellin Saldana

Group Leader
Marcellin Group

Professor Lars Nielsen

Senior Group Leader
Nielsen Group

Researchers Involved