Systems biology and metabolic engineering of cell factories  

I am part of the research groups led by Dr Esteban Marcellin and Prof Lars Nielsen. As part of the Queensland Strain Factory, we work in collaboration with Amyris, a renowned company and world leader in synthetic biology. We use systems biology to characterise yeast strains using proteomics and metabolomics techniques,  and ultimately implement a rational design of new strains with improved phenotypes. I am also involved in the engineering of mammalian cells and non-model organisms such as P. freudenreichii and Streptomyces using traditional engineering strategies and CRISPR/Cas9. 

I got a B. Eng. in Biotechnology and a MSc in Bioprocesses from the Instituto Politecnico Nacional (Mexico). In 2018, I completed my PhD at The University of Queensland, under the supervision of Dr. Esteban Marcellin and Prof. Lars Nielsen. My research project aimed the introduction of a metabolic pathway from Propionibacteria into Escherichia coli and Saccharomyces cerevisiae for the production of propionic acid and the erythromycin precursor 6-deoxyeritronolide B. To achieve that goal, I implemented the use of genome-scale modelling, design and construction of synthetic gene expression systems, evaluation of the performance of the recombinant strains in instrumented bioreactors, adaptive laboratory evolution, proteomics, metabolomics and next-generation sequencing.   

Key Publications

Gonzalez‐Garcia, R. A., McCubbin, T., Turner, M. S., Nielsen, L. K., & Marcellin, E. (2020). Engineering Escherichia coli for propionic acid production through the Wood–Werkman cycle. Biotechnology and bioengineering, 117(1), 167-183.  

Gonzalez-Garcia RA, McCubbin T, Wille A, Plan M, Nielsen LK, Marcellin E. Awakening sleeping beauty: production of propionic acid in Escherichia coli through the sbm operon requires the activity of a methylmalonyl-CoA epimerase. Microbial Cell Factories. 2017 Jul 17;16(1):121. 

Gonzalez-Garcia RA, McCubbin T, Navone L, Stowers C, Nielsen LK, Marcellin E. Microbial Propionic Acid Production. Fermentation. 2017 May 15;3(2):21. 

Gonzalez-Garcia RA, Aispuro-Castro R, Salgado-Manjarrez E, Aranda-Barradas J, Garcia-Peña EI. Metabolic pathway and flux analysis of H 2 production by an anaerobic mixed culture. International Journal of Hydrogen Energy. 2017 Feb 16;42(7):4069-82. 

González-García RA, Garcia-Peña EI, Salgado-Manjarrez E, Aranda-Barradas JS. Metabolic flux distribution and thermodynamic analysis of green fluorescent protein production in recombinant Escherichia coli: The effect of carbon source and CO^ sub 2^ partial pressure. Biotechnology and Bioprocess Engineering: BBE. 2013 Nov 1;18(6):104 

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