Safer gene-editing tools for Australian livestock and biotech industries

March 2021March 2024

Project Summary​

Human neurons destined for genome editing

Editing the genome of an organism in an efficient and safe fashion is critical for the livestock and biotechnology industries. While CRISPR-Cas9 has become the method of choice for genome editing, it is known to introduce unwanted "on-target" and "off-target" mutations, limiting its utility. To address this we created a novel genome editing platform technology termed Crackling-CAST that is almost 100% accurate, while retaining the
efficiency of the classical Cas9 system.This project will exemplify the capabilities of the novel gene targeting platform in cell types used by the biotechnology and livestock sectors, ensuring its global uptake by these industries and delivering significant economic benefits for Australia.


Project members

Professor Ernst J. Wolvetang

Senior Group Leader
Wolvetang Group
UQ-StemCARE Director

Dr Giovanni Pietrogrande

European Leukodystrophies Association (ELA) Fellow
Wolvetang Lab

Tahmina Tabassum

PhD student
Wolvetang Group