Using human induced pluripotent stem cells to model brain and ageing diseases, to perform functional genomics and develop novel regenerative medicine approaches

Induced pluripotent stem cells and direct reprogrammed cell types capture an individual’s genetic make-up and constitute attractive and versatile model systems for performing human functional genomics. We combine reprogramming, CRISPR-Cas9 assisted genome/ epigenome modification and 2D and 3D (organoid) culture approaches with advanced imaging (optogenetics) and genome interrogation platforms (single cell RNA seq) to understand the molecular processes underlying human brain diseases and ageing with the aim of designing personalized precision medicine approaches.

Professor Ernst Wolvetang obtained his PhD from the University of Amsterdam, continued his post-doctoral training at the Monash Institute for Reproduction and Development, before joining the Australian Stem Cell Centre. In 2008 he was recruited to the AIBN where he heads the Stem Cell Engineering Laboratory and co-directs the UQ Centre in Stem Cell Ageing and Regenerative Engineering. He is a leader in the derivation, genome editing and disease modelling with human induced pluripotent stem cells, is the inaugural director of “Cell Reprogramming Australia”, and is a principal investigator in the ARC Centre of Excellence “Stem Cells Australia”. He was awarded the 2014 LSQ regenerative medicine prize. Professor Wolvetang serves on the editorial board of six stem cell journals; is listed inventor on four patents in stem cell research and he serves as a scientific advisor to the Massimo Foundation.


Professor Wolvetang collaborates with leading clinical researchers of neurological diseases such as Prof Marjo van der Knaap (VU Amsterdam) and Dr Adeline Vanderver (Washington Children Hospital) as well as with leaders in the cell reprogramming and stem cell field such as Dr Miguel Esteban (Guangzhou Institutes of Biomedicine and Health) and Professor Martin Pera (Jackson laboratories Bar Harbor, ME USA). Professor Wolvetang engages with the company InterK on testing peptide therapeutics and with the Massimo Foundation, BrAshAT and the GLIA consortium on finding and testing novel therapeutics for hypo-myelination diseases and childhood ataxias. The UQ Centre in stem cell ageing and Regenerative Engineering established in July 2016, co-directed by Professor Wolvetang, aims to develop novel therapeutic approaches to overcome the decline of stem cells with age.

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Professor Wolvetang is the recipient of more than $3.5 million in grant funding in the past five years.

  • 2018-2021      Perry Cross Spinal Research Foundation CIA Ruitenberg, CIB Wolvetang, $400,000.
  • 2018–2020     CIA- Associate Professor Ernst Wolvetang, CIB Associate Professor Jessica Mar. Defective repair of neuronal activity-induced DNA double strand breaks: A novel pathogenic mechanism for neurodegeneration in ataxia-telangiectasia. NHMRC, $ 578,812
  • 2018-2021     CIA Prof. Geoff Faulkner, CIB Associate Professor Ernst Wolvetang.  Does L1 retrotransposition accentuate schizophrenia susceptibility and progression? NHMRC APP1138795, $725,585.
  • 2018                Prof. J. Cooper-White, Prof. Ernst Wolvetang, Dr Jana Vukovic, Dr Rodrigo Medeiros. ARC Linkage Infrastructure, Equipment and Facilities: ARC LIEF LE180100200. AutoStem: A high performance, automated stem cell bioengineering facility, $318,000.
  • 2017-2020      CIA Tam, CIB Wolvetang, CIC Cooper-White, CID  Ovchinnikov   Re-wiring a stem cell: Deciphering the molecular mechanism underpinning lineage propensity, NHMRC APP1127976, $ 855,780.
  • 2017-2021    CIA Lister, CIB Polo, CIC Wolvetang, CID Heng Leveraging genomics strategies to generate adult neurons from iPSCs and somatic cells, NHMRC APP1130168, $ 1,593,336.      
  • 2014-2017       CIA-Professor Ryan Lister, CIB- Associate Professor Ernst Wolvetang, CIC-Dr Oz Bogdanovic. Deciphering the role of atypical DNA methylation in neural genome regulation and neurological disorders, APP1090116, $751,116.
  • 2016-2016       Elucidating Asp-tRNA synthetase deficiency.  Massimo Foundation $50k 
  • 2014-2017       CIA- Associate Professor Ernst Wolvetang. Targeting cerebellar degeneration in Ataxia-Telangiectasia using induced pluripotent stem cells. BrAshat grant $375,400.
  • 2014-2016       CIA- Associate Professor Ernst Wolvetang CIB- Associate Professor Christine Wells. Understanding Alzheimer's disease using Down syndrome as a model. University of Queensland NHMRC APP1062802 $756,894.      
  • 2013-2015       CIA - Professor Melissa Little CIB - Associate Professor Ernst Wolvetang CIC - Professor Justin Cooper-White. Directed differentiation of human embryonic stem cells to kidney progenitors. University of Queensland. NHMRC APP1041277. $ 630.158.
  • 2013-2016       CIA - Associate Professor Ernst Wolvetang CIB -Doctor Ryan Taft CIC - Professor Merlin Butler. Investigation of processed snoRNAs as cryptic regulators of the imprinted Prader-Willi syndrome locus.  University of Queensland. NHMRC APP1043023.  $ 650,802
  • 2011-2018       22 Investigators including Wolvetang E
    ARC Special Research Initiative in Stem Cell Science, Stem Cells Australia. (University of Melbourne,  Monash University, University of Queensland, University of New South Wales, Walter and Eliza Hall Institute & CSIRO). $21 million
  • 2012-2015       Lavin M , Wolvetang E
    Reprogramming of Ataxia-Telangiectaxia fibroblasts to generate iPS cells University of Queensland. NHMRC APP1021005 $ 581,010.00


Key Publications

Wolvetang E, Herszfeld D, Langton-Bunker E, Chung T, Filipczyk A, Houssami S, Koh K, Laslett AL, Michalska A, Nguyen L, Reubinoff BE, Tellis I, Auerbach JM, Ording CJ, Looijenga LHJ, Pera MF. (2006) CD30 is a survival factor and a biomarker for transformed human pluripotent stem cells. Nat Biotech 24(3), 351-357.

Nayler SP, Gatei M, Kozlov S, Gatti R, Mar JC, Wells CA, Lavin M, Wolvetang EJ. (2012) Induced pluripotent stem cells from ataxia-telangiectasia recapitulate the cellular phenotype. Stem cells Translational Medicine 1, 523-535

Chung T-L, Brena RM, Kolle G, Grimmond SM, Berman BP, Laird PW, Pera MF, Wolvetang EJ. (2010) Vitamin C promotes widespread yet specific demethylation of the hESC epigenome. Stem Cells 28(10), 1848-1855.

Minoru Takasato, Pei X Er, Han Chiu, Barbara Maier, Gregory J Baillie, Charles Ferguson, Robert G Parton, Ernst Wolvetang, Mathias S Roost, Susana M Chuva de Sousa Lopes, Melissa H Little Kidney organoids from human iPS cells contain multiple cell types and model human nephrogenesis (2015) Nature 526, 564–568.

Sabine Klawitter, Nina V. Fuchs, Kyle R. Upton, Martin Muñoz-Lopez4, Ruchi Shukla, Jichang Wang, Marta Garcia-Canadas, Daniel J. Gerhardt, Johannes Löwer, Ivana Grabundzija, Attila Sebe, Balázs Sarkadi, Sylvia Merkert, Anett Witthuhn, Alexandra Haase, Ernst J. Wolvetang, Ulrich Martin, Zoltán Ivics, Zsuzsanna Izsvák, Jose L. Garcia-Perez, Geoffrey J. Faulkner and Gerald G. Schumann. Reprogramming triggers endogenous L1, Alu and SVA retrotransposition in human induced pluripotent stem cells. (2016) Nature Comm 7.

Olivier J. Becherel, Jane Sun,  Abrey J. Yeo, Sam Nayler, Brent  L. Fogel, Fuying Gao, Giovanni Coppola, Chiara Criscuolo, Giuseppe De Michele, Martin F. Lavin*and Ernst Wolvetang*. A new model to study neurodegeneration in Ataxia Oculomotor Apraxia Type 2. Hum.Mol Gen.  (2015) ddv296

Briggs JA, Sun J, Shepherd J, Ovchinnikov DA, Chung TL, Nayler SP, Kao LP, Morrow CA, Thakar NY, Soo SY, Peura T, Grimmond SM, Wolvetang EJ. (2012) Integration-free iPS cells identify genetic and neural developmental features of Down syndrome etiology. Stem Cells 31(3), 467-478.