Super-resolution Imaging Study on Host-targeted Interventions Against Orthoflavivirus Neuroinvasion

This project addresses the growing threat of mosquito-borne orthoflaviviruses, including West Nile, Dengue and Usutu viruses, which are emerging public health risks in Australia and globally. As part of the REFINE consortium, we aim to identify new therapeutic targets and develop advanced human models to test the efficacy and safety of antiviral strategies, including antibodies, small-molecule inhibitors and vaccine candidates.

Our approach combines state-of-the-art virology, stem cell biology and advanced imaging. We generate virus-like particles and engineered reporter viruses to study how these pathogens infect human cells and spread within tissues. Using human skin models and induced pluripotent stem cell–derived neurons, we investigate key steps of viral infection, including long-range transport along neurons using custom microfluidic systems. Genetic and pharmacological tools are used to evaluate antiviral candidates, with high-resolution imaging enabling detailed analysis of virus–host interactions.

Together with the REFINE consortium, this work will advance understanding of neurotropic viral infections and accelerate the development of effective antiviral therapies, strengthening Australia’s preparedness for emerging infectious diseases.