Exosomes, extracellular vesicles (EVs) carrying cellular molecular contents and tissue-specific signaling molecules (e.g., DNA, exosomal miRNA, lipids, and cell-surface proteins), can be precisely and ultrasensitively detected in biological fluids. This project endeavors to create innovative nanotechnologies and nanofabrication strategies, resulting in a highly sensitive and robust nanoarchitectonics integrated automated platform for the molecular profiling of exosomes at a single-particle resolution. The developed technologies will offer insights into synthesizing target-specific mesoporous nanomaterials, nanofabrication strategies, and a nano-platform for the automatic isolation and quantification of exosomes and their contents, eliminating the need for sophisticated laboratories and human intervention. By combining mesoporous nanostructure design with project informatics, this project seeks to advance knowledge in nanoengineering, nanofabrication, and signal transduction, ultimately contributing to the field of exosome chemistry.
